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KMID : 0352720080320020107
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Journal of Ginseng Research
2008 Volume.32 No. 2 p.107 ~ p.113
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Inhibitory Effects of Panaxatriol from Panax ginseng C. A. Meyer on Phosphoinositide Breakdown Induced by Thrombin in Platelets
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Park Kyeong-Mee
Rhee Man-Hee
Shin Han-Jae
Song Yong-Bum
Hyun Hak-Chul
Park Ki-Hyun
Cho Hyun-Jeong
Choi Sun-A
Kang Hyo-Chan
Kim Gyung-Jin
Kim Hyeong-Soo
Kang Hee-Jin
Ok Woo-Jeong
Lee Dong-Ha
Park Hwa-Jin
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Abstract
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In this study, we have investigated the effect of panaxatriol (PT) on phosphoinositides (PIS) breakdown and -elevation in thrombin-induced platelet aggregation. Thrombin (5U/ml), a potent platelet agonist which activates phospholipase via protease activated receptor (PAR), hydrolyzed PIS in platelet membrane. The phosphatidylinositol 4, 5-bisphosphate was hydrolyzed after 10 sec of the thrombin-stimulation, and both the phosphatidylinositol 4-monophosphate (PIP) and phosphatidylinositol (PI) were brokendown after 30 sec of the thrombin-stimulation. However, PT inhibited the thrombin-stimulated hydrolysis of , PIP, and PI. On the other hand, thrombin increased the level of phosphatidic acid (PA) which is phosphorylated from diacylglycerol (DG) generated by PIS-hydrolysis. However, Pr inhibited the thrombin-increased PA level non-significantly. Thrombin increased cytosolic free ) up to 72% as compared with control in intact platelet. However, PT (100 ) inhibited the thrombin-elevated to 100%. These results suggest that PT may have a beneficial effect on platelet aggregation-mediated thrombotic disease by inhibiting thrombin-induced platelet aggregation via suppression of the level and PIS breakdown.
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KEYWORD
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Panaxatriol, Cytosolic free Ca 2+, Phosphoinositides, Phosphatidic acid
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